Quick Tutorial

Download the example data here, and then follow the steps in this video to model the data using a two-state global HMM.

This HDF5 file contains two smFRET datasets in the single-molecule dataset (SMD) format. They are two different smFRET signals (L1-L9 and L1-tRNA) on the same bacterial ribosome complex with deactylated tRNA\(^{Phe}\) in the P-site at 25. These data are published in a recent paper 1.

Footnotes

  1. Ray, K., Kinz-Thompson, C.D., Fei, J., Wang, B., Lin, Q., Gonzalez, Jr., R.L. (2023) Entropic control of the free-energy landscape of an archetypal biomolecular machine. Proc. Natl. Acad. Sci. USA, 120(21), e2220591120. DOI: 10.1073/pnas.2220591120.↩︎